Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney transfected with LFABP)
Loading amount
15 µg
Specification
Kidney transfected with LFABP
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
odyssey
Exposure
50 second(s)
Bands
Specific: 70 kDa
Negative control
human kidney cells
Additional data
Additional Notes
band 1 = Human kidney lysate
band 2 = transfected kidney lysate
2nd picture = weaker exposure to identify bands
band 2 = transfected kidney lysate
2nd picture = weaker exposure to identify bands
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Valentina Marcelli
Verified customer
投稿 Jun 11 2010