Application
Western blot
Sample
Mouse Cell lysate - whole cell (J774 mouse macrophages, Human fibroblasts)
Loading amount
50000 cells
Specification
J774 mouse macrophages, Human fibroblasts
Treatment
lysis with LDS sample buffer, DTT, 70C for 10 min.
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (whole molecule)–Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 47 kDa Non-specific: 38, 36 kDa
Positive control
pure LAL antibody, 10ng
Additional data
Additional Notes
Lanes: 1: Purified LAL antibody. 2: human monocyte-derived macrophages (lysate)
3&4: Human fibroblasts (lysate) 5: J774 macrophages (lysate)
EXPECTED BAND SIZE: 45-47MW
3&4: Human fibroblasts (lysate) 5: J774 macrophages (lysate)
EXPECTED BAND SIZE: 45-47MW
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 15 2007