Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Preparation
Cell harvesting/tissue preparation method: mechanical disruption of spleen
Sample buffer: 2% FCS, 0.5 mM EDTA in PBS
Sample buffer: 2% FCS, 0.5 mM EDTA in PBS
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
lymphocytes
Other product details
Dilution
1/100
Incubation time
30 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% FCS, 0.5 mM EDTA in PBS
Secondary antibody
Secondary antibody
None used
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投稿 Sep 19 2012