Application
Western blot
Sample
Human Cell lysate - whole cell (human sebacous gland cells)
Loading amount
60 µg
Specification
human sebacous gland cells
Treatment
13-cis Retinoic acid for 48 hours
Gel Running Conditions
Reduced Denaturing (4- 12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat dry milk in TBS-T (0.05%Tween
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti Mouse HRP-linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
West Pico ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Additional data
PubMed ID
18317594: View
Additional Notes
Image Notes
SEB-1 sebocytes total cellular protein
Lane 1-vehicle control
Lane 2- 0.1 uM 13-cis RA
Lane 3- 1uM 13-cis RA
SEB-1 sebocytes total cellular protein
Lane 1-vehicle control
Lane 2- 0.1 uM 13-cis RA
Lane 3- 1uM 13-cis RA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 09 2008