Anti-Leptin Receptor 抗体 (ab50424)
Key features and details
- Goat polyclonal to Leptin Receptor
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Leptin Receptor antibody
Leptin Receptor 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Leptin Receptor -
由来種
Goat -
特異性
This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1) of Leptin Receptor. -
アプリケーション
適用あり: IHC-P, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog -
免疫原
Synthetic peptide corresponding to Human Leptin Receptor aa 829-841 (internal sequence).
Sequence:C-TQDDIEKHQSDAG
Database link: P48357 -
ポジティブ・コントロール
- WB: Human Cerebellum, Mouse Foetal Brain and Rat Brain lysate. Flow Cyt: K562 cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50424の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
---|
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway. -
組織特異性
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain. -
ドメイン
The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 450207 Dog
- Entrez Gene: 3953 Human
- Entrez Gene: 16847 Mouse
- Entrez Gene: 24536 Rat
- Omim: 601007 Human
- SwissProt: P48357 Human
- SwissProt: P48356 Mouse
- SwissProt: Q62959 Rat
see all -
別名
- CD 295 antibody
- CD295 antibody
- CD295 antigen antibody
see all
画像
-
Lane 1 : Anti-Leptin Receptor antibody (ab50424) at 1.75 µg/ml
Lanes 2-3 : Anti-Leptin Receptor antibody (ab50424) at 1.5 µg/ml
Lane 1 : Human Cerebellum tissue lysate
Lane 2 : Mouse Foetal Brain tissue lysate
Lane 3 : Rat Brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 132 kDaLysate in RIPA buffer. Detected by chemiluminescence.
-
Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab50424 は 1 報の論文で使用されています。
- Maranesi M et al. Leptin System in Obese Dog Skin: A Pilot Study. Animals (Basel) 10:N/A (2020). PubMed: 33316917