製品の概要

  • 製品名
    Anti-Laminin beta 1 antibody
    Laminin beta 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Laminin beta 1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized peptide derived from the C-terminus of human Laminin beta 1.

  • ポジティブ･コントロール
    • Hela cells, human liver carcinoma tissue and extracts from HepG2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 60, 67, 110, 130, >170 kDa (predicted molecular weight: 198 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 配列類似性
    Contains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • ドメイン
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CLM antibody
      • Cutis laxa with marfanoid phenotype antibody
      • LAM B1 antibody
      • LAMB 1 antibody
      • LAMB1 antibody
      • LAMB1_HUMAN antibody
      • Laminin B1 antibody
      • Laminin B1 chain antibody
      • Laminin beta 1 chain antibody
      • Laminin beta 1 chain precursor antibody
      • Laminin beta1 antibody
      • Laminin subunit beta 1 antibody
      • Laminin subunit beta-1 antibody
      • Laminin-1 subunit beta antibody
      • Laminin-10 subunit beta antibody
      • Laminin-12 subunit beta antibody
      • Laminin-2 subunit beta antibody
      • Laminin-6 subunit beta antibody
      • Laminin-8 subunit beta antibody
      • LIS5 antibody
      • MGC142015 antibody
      see all

    画像

    • Western blot - Laminin beta 1 antibody (ab69633)
      Western blot - Laminin beta 1 antibody (ab69633)
      All lanes : Anti-Laminin beta 1 antibody (ab69633) at 1/500 dilution

      Lane 1 : Extracts from HepG2 cells
      Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 198 kDa
      Observed band size: >170 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 110 kDa, 130 kDa, 60 kDa, 67 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin beta 1 antibody (ab69633)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin beta 1 antibody (ab69633)
      Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69633 at 1:50 dilution.
      Left image un-treated.
      Right image treated with immunizing peptide.
    • Immunocytochemistry/ Immunofluorescence - Laminin beta 1 antibody (ab69633)
      Immunocytochemistry/ Immunofluorescence - Laminin beta 1 antibody (ab69633)
      Immunofluorescence analysis of HeLa cells using ab69633 at a 1:500 dilution.
      Left image un-treated.
      Right image treated with immunizing peptide.

    参考文献

    This product has been referenced in:
    • Cheng YC  et al. Candidate tumor suppressor B-cell translocation gene 3 impedes neoplastic progression by suppression of AKT. Cell Death Dis 6:e1584 (2015). Read more (PubMed: 25569101) »

    See 1 Publication for this product

    ab69633 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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