Anti-Laminin 抗体 (ab11575)
Key features and details
- Rabbit polyclonal to Laminin
- Suitable for: Dot blot, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Laminin antibody
Laminin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Laminin -
由来種
Rabbit -
特異性
In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C. -
アプリケーション
適用あり: Dot blot, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Dog, Pig, Xenopus laevis, Mammals, Amphibian -
免疫原
Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.
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特記事項
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.027% PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11575の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
1/1000. This concentration was determined using laminin at 50 ng per dot.
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IHC-P | (27) |
Use at an assay dependent concentration.
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特記事項 |
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Dot blot
1/1000. This concentration was determined using laminin at 50 ng per dot. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
組織特異性
Broadly expressed in: skin, heart, lung, and the reproductive tracts. -
配列類似性
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
ドメイン
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain IV is globular. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10319 Human
- Entrez Gene: 284217 Human
- Entrez Gene: 3908 Human
- Entrez Gene: 3912 Human
- Entrez Gene: 3913 Human
- Entrez Gene: 3915 Human
- Entrez Gene: 3918 Human
- Entrez Gene: 16772 Mouse
see all -
別名
- LAMA 1 antibody
- LAMA antibody
- LAMA1 antibody
see all
画像
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Immunohistochemical analysis of formalin fixed paraffin-embedded human tongue sections labeling Laminin with ab11575 at 1/30 dilution. Goat Anti-Rabbit IgG, FITC conjugate was used as secondary antibody.
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Immunohistochemical analysis of formalin fixed paraffin-embedded human tonsil sections labeling Laminin with ab11575 at 1/50 dilution. Goat Anti-Rabbit IgG, Cy3™ conjugate was used as secondary antibody.
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Immunoblotting
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate.
Lanes
1. antibody dilution 1/1,000
2. antibody dilution 1/2,000
3. antibody dilution 1/4,000
4. antibody dilution 1/8,000
5. antibody dilution 1/16,000
6. antibody dilution 1/32,000
7. Negative control: without primary antibody -
This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (567)
ab11575 は 567 報の論文で使用されています。
- Mesquita FCP et al. Decellularized Extracellular Matrix Powder Accelerates Metabolic Maturation at Early Stages of Cardiac Differentiation in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Cells Tissues Organs 212:32-44 (2023). PubMed: 34933302
- Song MJ et al. Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration. Nat Methods 20:149-161 (2023). PubMed: 36550275
- Adegoke MA et al. Flexibility of in vitro cortical circuits influences resilience from microtrauma. Front Cell Neurosci 16:991740 (2022). PubMed: 36589287
- Xu J et al. Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling. Am J Cancer Res 12:5552-5563 (2022). PubMed: 36628278
- Rostami S et al. Evaluation of Early Markers of Ischemia-reperfusion Injury and Preservation Solutions in a Modified Hindlimb Model of Vascularized Composite Allotransplantation. Transplant Direct 8:e1251 (2022). PubMed: 34912943