Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (grafted human skin)
Specification
grafted human skin
Fixative
Acetone
Blocking step
10% normal goat serum + 0.1% BSA in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: F(ab')2 fragment goat anti-rabbit Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
We used laminin 5 antibodies from other companies, but none of them worked. Your antibody worked well. We will buy it again.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Cynthia Simbulan-Rosenthal
Verified customer
投稿 Jun 28 2007