Application
Western blot
Sample
Human Cell lysate - whole cell (Breast)
Loading amount
10 µg
Specification
Breast
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
15 second(s)
Bands
Specific: alpha 3 chain - 165 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Yoon Pyo Choi
Verified customer
投稿 Jun 25 2009