Anti-Lamin B2 抗体 [LN43] (ab8983)
Key features and details
- Mouse monoclonal [LN43] to Lamin B2
- Suitable for: Flow Cyt, IHC-Fr, WB, IP
- Reacts with: Human, Zebrafish
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Lamin B2 antibody [LN43]
Lamin B2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [LN43] to Lamin B2 -
由来種
Mouse -
特異性
This antibody reacts with an epitope located in the C-terminal part of lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. Please see the listed positive controls for this antibody. Other cell/tissue types have not been tested. -
アプリケーション
適用あり: Flow Cyt, IHC-Fr, WB, IPmore details -
種交差性
交差種: Human, Zebrafish -
免疫原
Tissue, cells or virus corresponding to Lamin B2. Detergent insoluble fraction of potoroo cell line PtK1.
Database link: Q03252 -
エピトープ
Epitope located in the C-terminal part of lamin B2. -
ポジティブ・コントロール
- IHC-Fr: testicular germ cell tumour sections (see Machiels et al 1997), parabasal cells of skin epidermis (see Broers et al). WB: NCI-H125 nuclear cell lysate Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
LN43 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8983の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr |
1/1.
(when used with an avidin-biotinylated HRP complex as detection agent). |
|
WB | (2) |
1/100 - 1/1000. Detects a band of approximately 65-67 kDa.
|
IP |
Use at an assay dependent concentration.
|
特記事項 |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/1. (when used with an avidin-biotinylated HRP complex as detection agent). |
WB
1/100 - 1/1000. Detects a band of approximately 65-67 kDa. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
関連疾患
Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
細胞内局在
Nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 84823 Human
- Omim: 150341 Human
- SwissProt: Q03252 Human
- Unigene: 728836 Human
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別名
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
see all
画像
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All lanes : Anti-Lamin B2 antibody [LN43] (ab8983) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
ab8983 immunohistochemistry on a frozen section of human kidney showing nuclear lamina staining in the ductal epithelium.
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IHC-Fr of human kidney showing nuclear lamina staining in the ductal epithelium.
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Lamin B2 - Nuclear Envelope Marker was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Lamin B2 - Nuclear Envelope Marker (ab8983) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8983.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 73kDa: Lamin B2 -
Overlay histogram showing HeLA cells stained with ab8983 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8983, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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IF staining of a 9 days old zebrafish embryo.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (42)
ab8983 は 42 報の論文で使用されています。
- Hauser N et al. Exploiting the Endogenous Ubiquitin Proteasome System in Targeted Cancer Treatment. Cancers (Basel) 15:N/A (2022). PubMed: 36612252
- Madakashira BP et al. Nuclear Organization during Hepatogenesis in Zebrafish Requires Uhrf1. Genes (Basel) 12:N/A (2021). PubMed: 34356097
- Zhao X et al. Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway. J Ophthalmol 2021:6694050 (2021). PubMed: 33628480
- Schep R et al. Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance. Mol Cell 81:2216-2230.e10 (2021). PubMed: 33848455
- Zhou Y et al. MicroRNA-9-3p Aggravates Cerebral Ischemia/Reperfusion Injury by Targeting Fibroblast Growth Factor 19 (FGF19) to Inactivate GSK-3ß/Nrf2/ARE Signaling. Neuropsychiatr Dis Treat 17:1989-2002 (2021). PubMed: 34177264