Application
Western blot
Sample
Human Cell lysate - nuclear (NCI-H929)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
200000 cells
Specification
NCI-H929
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: HRP-linked anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Apr 20 2012