Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Lens Epithelial Cells)
Specification
Human Lens Epithelial Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X 100 in 1X PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: FITC AffiniPure Donkey Anti Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Counterstained with DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Andrea Caceres
Verified customer
投稿 Aug 04 2008