Application
Western blot
Sample
Pig Cell lysate - nuclear (fibroblasts)
Gel Running Conditions
Reduced Denaturing (10% Tris-glycine)
Loading amount
40 µg
Specification
fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk, 0.1% Tween, PBS
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: P0447
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 78, 70 kDa
Positive control
primary porcine fibroblast nuclear lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Maeve Ballantyne
Verified customer
投稿 Dec 22 2015