Application
Western blot
Sample
Cow Cell lysate - whole cell (VVEC)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Treatment
ATP 100 uM TC
Specification
VVEC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% BSA
Dilution
1/2000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
no treatment
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 34 kDa Non-specific: none kDa
Positive control
treatment
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 21 2016