Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Adherent cells, Cell culture media, Other biological fluids, Plasma, Serum, Suspension cells, Tissue Extracts, Urine
- Sensitivity: 1 uU/ml
製品の概要
-
製品名
Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)
Lactate Dehydrogenase キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Adherent cells, Suspension cells, Tissue Extracts, Cell culture media -
アッセイタイプ
Enzyme activity -
検出感度
1 uU/ml -
全工程の試験時間
0h 20m -
種交差性
交差種: Mammals, Other species -
製品の概要
Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples. In this assay, LDH converts lactate into pyruvate and NADH, which reacts with the specific fluorescent probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).
This kit is simple, highly sensitive and high-throughput adaptable and can detect LDH activity as low as 1 µU/mL.
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K730 PicoProbe™ Lactate Dehydrogenase Activity Assay Kit. K730-500 is the same size as the 500 test size of ab197000.
Lactate dehydrogenase (LDH, L-Lactate NAD oxidoreductase, EC 1.1.1.27) is an ubiquitous enzymes among vertebrate organisms which catalyzes the reversible conversion of pyruvate to lactate, with concomitant conversion of NADH and NAD+. LDH is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries.
-
試験プラットフォーム
Microplate reader
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 500 tests 500 tests Developer Mix I 1 vial 1 vial LDH Assay Buffer 1 x 110ml 1 x 110ml LDH Positive Control 1 vial 1 vial NADH Standard I 1 vial 1 vial PicoProbe (1.5 ml) 1 x 1.4ml 1 x 1.4ml -
研究分野
-
パスウェイ
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
関連疾患
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
配列類似性
Belongs to the LDH/MDH superfamily. LDH family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm. - Information by UniProt
-
別名
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all -
参照データベース
- SwissProt: Q7SI97 Other species
関連製品
-
Assay kits
画像
-
Typical NADH Standard Curve obtained following assay protocol.
-
Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.
-
Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (13)
ab197000 は 13 報の論文で使用されています。
- Pyun J et al. Copper bis(thiosemicarbazone) complexes modulate P-glycoprotein expression and function in human brain microvascular endothelial cells. J Neurochem 162:226-244 (2022). PubMed: 35304760
- Nimiritsky P et al. Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism. Biomedicines 9:N/A (2021). PubMed: 34572378
- Visavadiya NP et al. Distinct glycolytic pathway regulation in liver, tumour and skeletal muscle of mice with cancer cachexia. Cell Biochem Funct 39:802-812 (2021). PubMed: 34129243
- Zhao Y et al. Clinical features of cardiac lymphoma: an analysis of 37 cases. J Int Med Res 49:300060521999558 (2021). PubMed: 33752450
- Jose A et al. Anti-proliferative potential of phytochemical fractions isolated from Simarouba glauca DC leaf. Heliyon 6:e03836 (2020). PubMed: 32373740