Lactate Dehydrogenase B / LDH-B Activity Assay (ab140361)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture supernatant, Cell Lysate, Plasma, Purified protein, Serum, Tissue Extracts
製品の概要
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製品名
Lactate Dehydrogenase B / LDH-B Activity Assay
Lactate Dehydrogenase B/LDH-B キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 3 5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 4 5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Adherent cells, Tissue Extracts, Cell Lysate, Purified protein -
アッセイタイプ
Enzyme activity -
検出範囲
8 ng/ml - 500 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 95 86% - 111% Cell culture media 94 91% - 101% -
種交差性
交差種: Goat, Cow, Human
非交差種: Mouse, Rat -
製品の概要
The microplate assay ab140361 is used to determine Lactate dehydrogenase B / LDH-B activity (LDHB) in a sample utilizing the immunocapture technique. The specific native LDHB enzyme is immune-captured within the wells of the microplate and activity is determined by following the production of NADH catalyzed by the enzyme. The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M-1 cm-1). The immune-capture based activity assay kit contains all the required reagents for a fast and simple measurement of LDHB activity in a whole cell extract, tissue homogenate, or serum.
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特記事項
Functional lactate dehydrogenase are homo or hetero tetramers composed of M and H protein subunits encoded by the LDHA and LDHB genes, respectively. The major isozymes of skeletal muscle and liver has four muscle (M) subunits; while the main isozymes for heart muscle contains four (H) subunits. The other variants contain both types of subunits. Elevated levels of LDH indicate tissue breakdown in hemolysis. LDH can be used as a marker of myocardial infarction, peaking at days 3 and lasting through day 10. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis, and HIV. Defects in LDHB are a cause of hereditary LDHB deficiency; however LDHB deficiency is usually asymptomatic.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X Coupler 1 unit 100X NAD+ 1 unit 100X Reagent Dye 1 unit 10X Blocking Buffer 1 x 6ml 10X Buffer 1 x 25ml 1X LDH-B Control 1 x 1µg Extraction Buffer (ab260490) 1 x 15ml LDH-B Microplate 1 unit Reaction Base Buffer 1 x 10ml -
研究分野
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パスウェイ
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
関連疾患
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
配列類似性
Belongs to the LDH/MDH superfamily. LDH family. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- Epididymis secretory protein Li 281
- HEL S 281
- L lactate dehydrogenase B chain
see all -
参照データベース
- Entrez Gene: 281275 Cow
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: Q5E9B1 Cow
- SwissProt: P07195 Human
- Unigene: 446149 Human
関連製品
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Positive Controls
画像
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Example control standard curve generaated using the method described in the protocl booklet.
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LDHB activity was measured from Hela cell lysate, which contains hetero-tetramer isozyme composed of both (M) LDHA and (H) LDHB subunits. However, the liver derived cell line, HepG2, which contains mainly homo-tetramer of subunit M (LDHA), shows no enzymatic activity.
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The species cross reactivity of this assay was demonstrated by testing the sera from multiple species. The kit shows a good reaction with human, goat and bovine samples, but shows no reaction with mouse or rat sera which may also contain LDHB. This species cross reaction specificity is due to the species specificity of the capture antibody.
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Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: LDHA (purified from rabbit skeletal muscle)
Lane 4: LDHB (recombinant)
Lane 5: HeLa – In-well extracted with capture mAb
Lane 6: HepG2 – In-well extracted with capture mAb
Lane 7: LDHA – In-well extracted with a capture mAb
Lane 8: LDHB – In-well extracted with capture mAbThe western blot results indicate that the capture antibody isolates active LDHB, but does not isolate LDHA (which would be detected by the anti-LDH antibody).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab140361 は 3 報の論文で使用されています。
- Deng H et al. Targeting lactate dehydrogenase B-dependent mitochondrial metabolism affects tumor initiating cells and inhibits tumorigenesis of non-small cell lung cancer by inducing mtDNA damage. Cell Mol Life Sci 79:445 (2022). PubMed: 35877003
- Padilla J et al. A Heme-Binding Transcription Factor BACH1 Regulates Lactate Catabolism Suggesting a Combined Therapy for Triple-Negative Breast Cancer. Cells 11:N/A (2022). PubMed: 35406740
- Song T et al. Antibodies against Clonorchis sinensis LDH could cross-react with LDHB localizing on the plasma membrane of human hepatocarcinoma cell SMMC-7721 and induce apoptosis. Parasitol Res 115:1595-603 (2016). PubMed: 26769711