LY 294002, PI3-kinase inhibitor (ab120243)
Key features and details
- PI3-kinase inhibitor
- CAS Number: 154447-36-6
- Purity: > 99%
- Soluble in DMSO to 25 mM and in ethanol to 5 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
-
製品名
LY 294002, PI3-kinase inhibitor -
製品の詳細
PI3-kinase inhibitor -
生理活性の詳細
A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).
-
精製度
> 99% -
CAS 番号
154447-36-6 -
構造式
製品の特性
-
体系名
2-(4-Morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one -
分子量
307.35 -
分子式
C19H17NO3 -
PubChem 登録番号
3973 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 25 mM and in ethanol to 5 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES 線形表記
O=C2C=C(Oc1c(cccc12)c3ccccc3)N4CCOCC4 -
由来
Synthetic
-
研究分野
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
画像
-
2D chemical structure image of ab120243, LY 294002, PI3-kinase inhibitor
-
Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.
Lane 1: Marker
Lane 2: A431 lysate starved
Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)
Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution
Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.
Observed bands:
RSK1p90 (pS380) band size: 83 kDa
AKT1 (pS473) band size: 56 kDa
ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
RPS6 (pS235/236) band size: 31 kDa
Rab11a band size: 24 kDa
Unknown band: 130kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (40)
ab120243 は 40 報の論文で使用されています。
- Choi YA et al. IRF3 Activation in Mast Cells Promotes FcεRI-Mediated Allergic Inflammation. Cells 12:N/A (2023). PubMed: 37296614
- Kong F et al. Inhibition of apoptosis-regulatory protein Siva-1 reverses multidrug resistance in gastric cancer by targeting PCBP1. Oncol Res 30:277-288 (2022). PubMed: 37303491
- Jiang T et al. Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide. Tissue Eng Regen Med 19:403-415 (2022). PubMed: 35122584
- Liu S et al. Protection of Human Lens Epithelial Cells from Oxidative Stress Damage and Cell Apoptosis by KGF-2 through the Akt/Nrf2/HO-1 Pathway. Oxid Med Cell Longev 2022:6933812 (2022). PubMed: 35222803
- Tanaka Y et al. Nectin cell adhesion molecule 4 regulates angiogenesis through Src signaling and serves as a novel therapeutic target in angiosarcoma. Sci Rep 12:4031 (2022). PubMed: 35256687