Application
Western blot
Sample
Rabbit Tissue lysate - whole (bone)
Loading amount
20 µg
Specification
bone
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Bovine anti-goat
Host species: Cow
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Cow
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
BCIP/NBT
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 51, 37 (GAPDH) kDa
Positive control
Rabbit Bone samples were used and probed at the same time with GAPDH as a loading control.
Additional data
Additional Notes
PVDF membrane was used in WB; secondary was bovine anti-goat from another vendor which incubated for 2 hours as well. Antibody buffer was 1% NFDM in TBS
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 17 2006