Application
Western blot
Sample
Guinea pig Tissue lysate - other (Brain)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
1.25 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% milk
Dilution
1/50000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Bio-rad
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 85 kDa
Additional data
Additional Notes
This antibody is very strong even when protein loading amount is at 1 ug. Additional dilution maybe required.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 04 2016