Anti-LOX 1 抗体 (ab60178)
Key features and details
- Rabbit polyclonal to LOX 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LOX 1 antibody
LOX 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LOX 1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human LOX 1 (N terminal).
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ポジティブ・コントロール
- WB Jurkat cells IHC-P: Human small intestines
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3659R LOX-1 Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60178の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (2) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 31 kDa. Proform detected at 50 kDa, mature form detected at 31 kDa.
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 31 kDa. Proform detected at 50 kDa, mature form detected at 31 kDa. |
ターゲット情報
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機能
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. -
組織特異性
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. -
関連疾患
Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. -
配列類似性
Contains 1 C-type lectin domain. -
ドメイン
The cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL. -
翻訳後修飾
The intrachain disulfide-bonds prevent N-glycosylation at some sites.
N-glycosylated. -
細胞内局在
Cell membrane. Secreted. A secreted form also exists. - Information by UniProt
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参照データベース
- Entrez Gene: 4973 Human
- Omim: 602601 Human
- SwissProt: P78380 Human
- Unigene: 412484 Human
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別名
- C-type lectin domain family 8 member A antibody
- CLEC8A antibody
- hLOX 1 antibody
see all
画像
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All lanes : Anti-LOX 1 antibody (ab60178) at 0.5 µg/ml
All lanes : Jurkat cell lysate
Predicted band size: 31 kDa
Observed band size: 31,50 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of formalin fixed, paraffin-embedded human small intestine vessels labelling LOX 1 with ab60178 at 5 µg/ml, followed by biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Heat-induced antigen retrieval.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (61)
ab60178 は 61 報の論文で使用されています。
- Liu H et al. Gypenoside L and Gypenoside LI Inhibit Proliferation in Renal Cell Carcinoma via Regulation of the MAPK and Arachidonic Acid Metabolism Pathways. Front Pharmacol 13:820639 (2022). PubMed: 35370678
- Tsumita T et al. The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells. Int J Cancer 151:944-956 (2022). PubMed: 35608341
- Rosa CM et al. Effects of the SGLT2 Inhibition on Cardiac Remodeling in Streptozotocin-Induced Diabetic Rats, a Model of Type 1 Diabetes Mellitus. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624845
- Wei X et al. Pharmacological inhibition of EZH2 by GSK126 decreases atherosclerosis by modulating foam cell formation and monocyte adhesion in apolipoprotein E-deficient mice. Exp Ther Med 22:841 (2021). PubMed: 34149887
- Murdocca M et al. Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34439368