Anti-LOX 1 抗体 [23C11] (ab81709)
Key features and details
- Mouse monoclonal [23C11] to LOX 1
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-LOX 1 antibody [23C11]
LOX 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [23C11] to LOX 1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human LOX 1 aa 50-300 (extracellular). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- IHC-P: human spleen cells. Human APCs
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特記事項
ab81709 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumor antigen using anti LOX 1 antibody ab81709 induces anti tumor immunity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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特記事項(精製)
Purified and 0.2 µm filtered solution. -
一次抗体 備考
ab81709 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumor antigen using anti LOX 1 antibody ab81709 induces anti tumor immunity. -
ポリ/モノ
モノクローナル -
クローン名
23C11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81709の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100.
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特記事項 |
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Flow Cyt
Use a concentration of 10 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. |
ターゲット情報
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機能
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. -
組織特異性
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. -
関連疾患
Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. -
配列類似性
Contains 1 C-type lectin domain. -
ドメイン
The cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL. -
翻訳後修飾
The intrachain disulfide-bonds prevent N-glycosylation at some sites.
N-glycosylated. -
細胞内局在
Cell membrane. Secreted. A secreted form also exists. - Information by UniProt
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参照データベース
- Entrez Gene: 4973 Human
- Omim: 602601 Human
- SwissProt: P78380 Human
- Unigene: 412484 Human
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別名
- C-type lectin domain family 8 member A antibody
- CLEC8A antibody
- hLOX 1 antibody
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画像
データシートおよび資料
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Datasheet download
参考文献 (8)
ab81709 は 8 報の論文で使用されています。
- Zhao T et al. Knockdown of OLR1 weakens glycolytic metabolism to repress colon cancer cell proliferation and chemoresistance by downregulating SULT2B1 via c-MYC. Cell Death Dis 13:4 (2021). PubMed: 34921134
- Li Q et al. HRD1 prevents atherosclerosis-mediated endothelial cell apoptosis by promoting LOX-1 degradation. Cell Cycle 19:1466-1477 (2020). PubMed: 32308114
- Choi S et al. Internalization and Transportation of Endothelial Cell Surface KCa2.3 and KCa3.1 in Normal Pregnancy and Preeclampsia. Oxid Med Cell Longev 2019:5820839 (2019). PubMed: 31871552
- Davis G et al. The effects of subacute inhaled multi-walled carbon nanotube exposure on signaling pathways associated with cholesterol transport and inflammatory markers in the vasculature of wild-type mice. Toxicol Lett 296:48-62 (2018). PubMed: 30081225
- Jacquemin C et al. Heat shock protein 70 potentiates interferon alpha production by plasmacytoid dendritic cells: relevance for cutaneous lupus and vitiligo pathogenesis. Br J Dermatol 177:1367-1375 (2017). PubMed: 28380264
- Li D et al. Targeting self- and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells. J Exp Med 209:109-21 (2012). IHC ; Human . PubMed: 22213806
- Jeannin P et al. Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors. Immunity 22:551-60 (2005). ICC/IF, Flow Cyt ; Human . PubMed: 15894273
- Delneste Y et al. Involvement of LOX-1 in dendritic cell-mediated antigen cross-presentation. Immunity 17:353-62 (2002). Human . PubMed: 12354387