Application
Western blot
Sample
Human Cell lysate - whole cell (Healthy and CLL B-cells)
Loading amount
40 µg
Specification
Healthy and CLL B-cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/200
Incubation time
7 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP,Santa Cruz biotechnology
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
Thermo scientific super-signal west pico
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 33 kDa, 68 kDa kDa
Positive control
LMW Kininogen protein (Human) (ab91118)
Additional data
Additional Notes
Lane 1-4 Healthy isolated B-lymphocytes
Lane 5-7 Chronic Lymphocytic Leukaemia (CLL) isolated B-lymphocytes
Lane 10-11 LMW Kininogen protein (Human) (ab91118) 0.02 ug
Image 2 LMW Kininogen protein (Human) (ab91118) 0.01 ug
Lane 5-7 Chronic Lymphocytic Leukaemia (CLL) isolated B-lymphocytes
Lane 10-11 LMW Kininogen protein (Human) (ab91118) 0.02 ug
Image 2 LMW Kininogen protein (Human) (ab91118) 0.01 ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Elena Kashuba
Verified customer
投稿 Apr 10 2013