Anti-LIMK2 (phospho T505) 抗体 (ab38499)
Key features and details
- Rabbit polyclonal to LIMK2 (phospho T505)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LIMK2 (phospho T505) antibody
LIMK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LIMK2 (phospho T505) -
由来種
Rabbit -
特異性
This antibody is specific for LIMK2 only when phosphorylated at threonine 505, but may also cross react with LIMK1 phosphorylated at tyrosine 505 based on homology. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human LIMK-2 (phospho T505).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38499の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa). |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. -
組織特異性
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated on serine and/or threonine residues by ROCK1. -
細胞内局在
Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3985 Human
- Omim: 601988 Human
- SwissProt: P53671 Human
- Unigene: 474596 Human
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別名
- LIM domain kinase 2 antibody
- LIM kinase 2 antibody
- LIMK 2 antibody
see all
画像
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All lanes : Anti-LIMK2 (phospho T505) antibody (ab38499) at 1/500 dilution
Lane 1 : MCF7 cell extract (5-30ug of total protein) pre-incubated with phosphopeptide.
Lane 2 : MCF7 cell extract (5-30ug of total protein).
Secondary
All lanes : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
ab38499 at a 1:50 dilution staining LIMK2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
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ICC/IF image of ab38499 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab38499 は 16 報の論文で使用されています。
- Fang N & Li P O-linked N-acetylglucosaminyltransferase OGT inhibits diabetic nephropathy by stabilizing histone methyltransferases EZH2 via the HES1/PTEN axis. Life Sci 274:119226 (2021). PubMed: 33609540
- Gu Y et al. Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203. Aging (Albany NY) 12:2333-2346 (2020). PubMed: 32019904
- Xiao Y et al. Circular RNA Involved in the Protective Effect of Malva sylvestris L. on Myocardial Ischemic/Re-Perfused Injury. Front Pharmacol 11:520486 (2020). PubMed: 33101012
- Sousa-Squiavinato ACM et al. Cofilin-1 signaling mediates epithelial-mesenchymal transition by promoting actin cytoskeleton reorganization and cell-cell adhesion regulation in colorectal cancer cells. Biochim Biophys Acta Mol Cell Res 1866:418-429 (2019). PubMed: 30296500
- Zhang Z et al. MicroRNA-493-5p inhibits proliferation and metastasis of osteosarcoma cells by targeting Kruppel-like factor 5. J Cell Physiol 234:13525-13533 (2019). PubMed: 30773645