Anti-LIM Kinase 1 抗体 [EPR912] (ab108507)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR912] to LIM Kinase 1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LIM Kinase 1 antibody [EPR912]
LIM Kinase 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR912] to LIM Kinase 1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- U-87 MG, SH SY5Y and Ramos cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR912 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108507の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 73 kDa. |
ターゲット情報
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機能
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. -
組織特異性
Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. -
関連疾患
Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
細胞内局在
Cytoplasm. Cell projection > growth cone. - Information by UniProt
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参照データベース
- Entrez Gene: 3984 Human
- Entrez Gene: 16885 Mouse
- Entrez Gene: 65172 Rat
- Omim: 601329 Human
- SwissProt: P53667 Human
- SwissProt: P53668 Mouse
- SwissProt: P53669 Rat
- Unigene: 647035 Human
see all -
別名
- EC 2.7.1.37 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: LIMK1 knockout HAP1 cell lysate (40 µg)
Lane 3: PC12 cell lysate (20 µg)
Lane 4: NIH3T3 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108507 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108507 was shown to specifically react with LIM Kinase 1 when LIM Kinase 1 knockout samples were used. Wild-type and LIM Kinase 1 knockout samples were subjected to SDS-PAGE. Ab108507 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab108507 は 5 報の論文で使用されています。
- Feng Y et al. Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis. Cell Mol Life Sci 79:498 (2022). PubMed: 36008729
- Pang D et al. Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-?B pathway. Biol Open 9:N/A (2020). PubMed: 31988091
- Zhu Q et al. IRX5 promotes colorectal cancer metastasis by negatively regulating the core components of the RHOA pathway. Mol Carcinog 58:2065-2076 (2019). PubMed: 31432570
- Duan X et al. The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis. J Cell Physiol 233:6088-6097 (2018). PubMed: 29319181
- Gu H et al. Genistein up-regulates miR-20a to disrupt spermatogenesis via targeting Limk1. Oncotarget 8:58728-58737 (2017). WB ; Mouse . PubMed: 28938591