Anti-LEF1 抗体 (ab85052)
Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LEF1 antibody
LEF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LEF1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
A synthetic peptide from intermediate residues of Human LEF1 protein, Swiss Prot Q9UJU2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 2% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85052の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/200 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 44 kDa).
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特記事項 |
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WB
1/200 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 44 kDa). |
ターゲット情報
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機能
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. -
組織特異性
Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. -
配列類似性
Belongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain. -
ドメイン
Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. -
細胞内局在
Nucleus. Found in nuclear bodies upon PIASG binding. - Information by UniProt
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参照データベース
- Entrez Gene: 51176 Human
- Entrez Gene: 161452 Rat
- Omim: 153245 Human
- SwissProt: Q9UJU2 Human
- SwissProt: Q9QXN1 Rat
- Unigene: 726506 Human
- Unigene: 21926 Rat
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別名
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab85052 は 6 報の論文で使用されています。
- Jiang C et al. NR4A1 promotes LEF1 expression in the pathogenesis of papillary thyroid cancer. Cell Death Discov 8:46 (2022). PubMed: 35110542
- Wang Q et al. Akt/mTOR integrate energy metabolism with Wnt signal to influence wound epithelium growth in Gekko Japonicus. Commun Biol 5:1018 (2022). PubMed: 36167813
- Aloysius A et al. The transcription factor Lef1 switches partners from ß-catenin to Smad3 during muscle stem cell quiescence. Sci Signal 11:N/A (2018). PubMed: 30042129
- Zhang T et al. Regulating osteogenesis and adipogenesis in adipose-derived stem cells by controlling underlying substrate stiffness. J Cell Physiol 233:3418-3428 (2018). PubMed: 28926111
- Jiang S et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep 14:1891-900 (2016). WB ; Human . PubMed: 27432616
- Meng Z et al. Tumorigenicity analysis of heterogeneous dental stem cells and its self-modification for chromosome instability. Cell Cycle 14:3396-407 (2015). PubMed: 26322910