Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Non-reduced Denaturing (7.5)
Loading amount
25 µg
Specification
liver
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 160, 130, 110 kDa
Additional data
Additional Notes
Works on mouse liver and human hepG2 cells
Several bands are observed by Wb because the LDLR has several post translational modifications.
Lane 1 : Mouse liver sample 1
Lane 2 : Mouse liver sample 2
Lane 3 : mouse liver sample 3
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 23 2016