Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
SH-SY5Y cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-mouse AF680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 34 kDa
Additional data
Additional Notes
Lane 1 is MW marker, lanes 2-5 are identical SH-SY5Y cell lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 19 2015