Anti-LC3B 抗体 (ab51520)
Key features and details
- Rabbit polyclonal to LC3B
- Suitable for: IHC-Fr, Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LC3B antibody
LC3B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LC3B -
由来種
Rabbit -
特異性
ab51520 is specific for the B isoform of LC3. -
アプリケーション
適用あり: IHC-Fr, Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to the N terminal region of human LC3, isoform B protein.
Database link: Q9GZQ8 -
ポジティブ・コントロール
- ICC/IF: HeLa, U373-MGc cells. IHC-P: U87-MG cells. Flow Cyt (Intra): NTERA-2 cells. IHC-P-Fr: U373-MG cells.
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特記事項
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
The product formulation changed to include 1% BSA on 17th May 2017. The following lot does not contain BSA and is still in stock GR320876. All future lots will contain 1% BSA.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51520の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-Fr | (2) |
1/2000.
|
Flow Cyt (Intra) |
1/200.
|
|
WB | (9) |
1/3000. Predicted molecular weight: 15 kDa. Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I).
|
ICC/IF | (3) |
1/2000.
|
特記事項 |
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IHC-Fr
1/2000. |
Flow Cyt (Intra)
1/200. |
WB
1/3000. Predicted molecular weight: 15 kDa. Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I). |
ICC/IF
1/2000. |
ターゲット情報
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機能
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
組織特異性
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. -
配列類似性
Belongs to the MAP1 LC3 family. -
翻訳後修飾
The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. -
細胞内局在
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 81631 Human
- Omim: 609604 Human
- SwissProt: Q9GZQ8 Human
- Unigene: 356061 Human
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別名
- ATG8F antibody
- Autophagy-related protein LC3 B antibody
- Autophagy-related ubiquitin-like modifier LC3 B antibody
see all
画像
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All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution
Lane 1 : Wild-type HepG2 untreated control cell lysate
Lane 2 : Wild-type HepG2 Treated Chloroquine (50 uM, 16 h) cell lysate
Lane 3 : MAP1LC3B knockout HepG2 untreated control cell lysate
Lane 4 : MAP1LC3B knockout HepG2 Treated Chloroquine (50 uM, 16 h) cell lysate
Lane 5 : U-87 MG cell lysate
Lane 6 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 14,16 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-LC3B antibody staining at 1/3000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab51520 was shown to bind specifically to LC3B. A band was observed at 16/14 kDa (yellow arrows) in treated wild-type HepG2 cell lysates with no signal observed at this size in MAP1LC3B knockout cell line ab277828 (knockout cell lysate ab283796). To generate this image, wild-type and MAP1LC3B knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
1) Control
2) Temozolomide -
All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution
Lane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 0 µM
Lane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 2 µM
Lane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 4 µM
Lane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 8 µM
Predicted band size: 15 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Flow cytometry (Intracellular) analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.
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Ab51520 at 1/2000 dilution staining treated U373-MG cells;
1) Control
2) C2-ceramide.
The nuclei were stained with DAPI. -
Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
1) Control
2) Temozolomide -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (399)
ab51520 は 399 報の論文で使用されています。
- Adamczyk-Grochala J et al. DNMT2/TRDMT1 gene knockout compromises doxorubicin-induced unfolded protein response and sensitizes cancer cells to ER stress-induced apoptosis. Apoptosis 28:166-185 (2023). PubMed: 36273376
- Peng F et al. AMPK/MFF Activation: Role in Mitochondrial Fission and Mitophagy in Dry Eye. Invest Ophthalmol Vis Sci 63:18 (2022). PubMed: 36374514
- Li W et al. Shikonin induces ferroptosis in multiple myeloma via GOT1-mediated ferritinophagy. Front Oncol 12:1025067 (2022). PubMed: 36387145
- Li W et al. Fasudil prevents neomycin-induced hair cell damage by inhibiting autophagy through the miR-489/NDP52 signaling pathway in HEI-OC1 cells. Exp Ther Med 23:43 (2022). PubMed: 34849158
- Ferreira CB et al. S327 phosphorylation of the presynaptic protein SEPTIN5 increases in the early stages of neurofibrillary pathology and alters the functionality of SEPTIN5. Neurobiol Dis 163:105603 (2022). PubMed: 34954322