Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cultured hippocampal astrocytes)
Specification
cultured hippocampal astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 for 5 min
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: AffiniPu Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5.5 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5.5 ®
Dilution
1/200
Additional data
Abcam response
Thank you for sending in your valuable feedback. This LAMP2b antibody has only been tested in human so far. The immunogen (from human LAMP2) has only 33% alignment to mouse LAMP2 and 66% alignment to mouse LAMP1. This may explain why the results are not clearer. We would recommend running a no-primary control to assess the amount of non-specific staining observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 16 2007