Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (BA9 - Cortical area)
Specification
BA9 - Cortical area
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk with PBS-T
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit Alexaflour 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/5000
Detection
Detection method
Odyssey
Bands
Specific: 45 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Nitrocellulose membrane, Tris based lysis buffer. Lanes key: 1 - rat cortical lysate 2 - human control cortical lysate 3 - human parkinson's dementia cortical lysate 4 - human dementia with lewy body cortical lysate, 5 - human Alzeheimer's disease cortical lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Martin Broadstock
Verified customer
投稿 Aug 16 2013