Application
Western blot
Sample
Human Cell lysate - whole cell (Cortical tissue)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Cortical tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk with PBS-T
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit Alexaflour 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45 and 80-120 kDa
Additional data
Additional Notes
Sample legends: 1 = rat cortex, 2 = Control human cortical tissue, 3 = Cortical tissue from patient with Parkinson's disease dementia, 4 = Cortical tissue from patient with Dementia with Lewy Bodies, 5 = Cortical tissue from patient with Alzheimer's disease.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Martin Broadstock
Verified customer
投稿 Aug 16 2013