Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (BA9 - Cortical area)
Specification
BA9 - Cortical area
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk with PBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit Alexaflour 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/5000
Detection
Detection method
Odyssey
Bands
Specific: 120 kDa Non-specific: range from 50-120 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Sample legends: 1 = rat cortex, 2 = Control human cortical tissue, 3 = Cortical tissue from patient with Parkinson's disease dementia, 4 = Cortical tissue from patient with Dementia with Lewy Bodies, 5 = Cortical tissue from patient with Alzheimer's disease.
Abcam response
Many thanks for your valuable feedback. It appears that under these experimental conditions ab125068 does not specifically detect Lamp2a. We welcome further customer feedback. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Martin Broadstock
Verified customer
投稿 Aug 19 2013