Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rat Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Positive control
Mouse glioblastoma (n2a) cell lysate
Additional data
Abcam response
Thank you for your valuable feedback about the lack of cross-reactivity of ab13524 with African green monkey, a species that we have not tested before. We appreciate the time and effort that the reviewer has put in to testing this product in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 16 2018