Application
Immunocytochemistry/ Immunofluorescence
Sample
Drosophila melanogaster Cell (larval fat body)
Permeabilization
No
Specification
larval fat body
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% Triton-X100 + 5%serum
Dilution
1/300
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Fixative: PFA 4%
Blocking buffer: PBS + 0.1% Triton-X100 + 5% Serum
Comment: no signal detected
Abcam response
We would like to thank this user for providing this additional data. At this time we have not done testing with ab30687 in ICC/IF. We always welcome any input our researchers might have on Abcam products and will continue to evaluate this antibody's performance in untested applications.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 19 2013