Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (WM3248 melanoma cell line)
Permeabilization
No
Specification
WM3248 melanoma cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Texas red goat anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Praveen Agrawal
Verified customer
投稿 Mar 24 2017