Application
Western blot
Sample
Rat Cell lysate - whole cell (Cortical neurons)
Loading amount
30 µg
Specification
Cortical neurons
Gel Running Conditions
Reduced Denaturing
Blocking step
Commercially available protein free blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Protein-free blocking reagent,0.1% tween
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 230 kDa Non-specific: 37 kDa
Positive control
The specific band was reduced in cells transfected with L1CAM siRNAs
Additional data
Additional Notes
Lane 1- untransfected neurons
Lane 2- neurons transfected with negative control siRNA
Lane 3 - neurons transfected with L1CAM siRNA
Lane 2- neurons transfected with negative control siRNA
Lane 3 - neurons transfected with L1CAM siRNA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 15 2008