Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: amb°C
Sample
Mouse Tissue sections (retina)
Specification
retina
Permeabilization
Yes - TBS-Tritòn X-100 0,025 % 15 min
Fixative
Methanol
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS buffer
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200
Additional data
Additional Notes
Blocking agent: 5 % normal goat serum + 0.3% Triton X-100 + 1 % BSA in TBS pH 7.4
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Patricia Bogdanov
Verified customer
投稿 Jul 15 2013