Anti-Ku70 抗体 [EPR4026] (ab108604)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4026] to Ku70
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Ku70 antibody [EPR4026]
Ku70 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4026] to Ku70 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- HeLa, HepG2, Raji, A673, A549 and A431 cell lysates; Human breast carcinoma tissue; HeLa cells
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4026 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108604の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
|
ICC/IF |
1/100 - 1/250.
|
特記事項 |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
-
機能
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. -
配列類似性
Belongs to the ku70 family.
Contains 1 Ku domain.
Contains 1 SAP domain. -
発生段階
Expression does not increase during promyelocyte differentiation. -
翻訳後修飾
Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 2547 Human
- Omim: 152690 Human
- SwissProt: P12956 Human
- Unigene: 292493 Human
-
別名
- 5''-deoxyribose-5-phosphate lyase Ku70 antibody
- 5''-dRP lyase Ku70 antibody
- 70 kDa subunit of Ku antigen antibody
see all
画像
-
All lanes : Anti-Ku70 antibody [EPR4026] (ab108604) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : A673 cell lysate
Lane 5 : A549 cell lysate
Lane 6 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa -
ab108604, at 1/100 dilution, staining Ku70 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab108604, at 1/100 dilution, staining Ku70 in HeLa cells by Immunofluorescence.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab108604 は 3 報の論文で使用されています。
- Agudelo JP et al. Multiparametric Magnetic Resonance Imaging and Metabolic Characterization of Patient-Derived Xenograft Models of Clear Cell Renal Cell Carcinoma. Metabolites 12:N/A (2022). PubMed: 36422257
- Castanotto D et al. A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus. Proc Natl Acad Sci U S A 115:E5756-E5765 (2018). PubMed: 29866826
- Lodillinsky C et al. p63/MT1-MMP axis is required for in situ to invasive transition in basal-like breast cancer. Oncogene 35:344-57 (2016). PubMed: 25893299