Application
Western blot
Sample
Human Cell lysate - whole cell (Raji, CLL B-lymphocytes,T47D,SW837)
Loading amount
20 µg
Specification
Raji, CLL B-lymphocytes,T47D,SW837
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/750
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP,Santa Cruz biotechnology
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
Thermo scientific super-signal west pico
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 33, 42, 55, 100 kDa
Positive control
Raji cell line
Additional data
Additional Notes
Lane 1-2 Raji cells
Lane 3-8 CLL isolated B-lymphocytes
Lane 9-10 T47D ( (Human ductal breast epithelial tumor cell line)
Lane 11-12 SW837 (Human Caucasian rectum adenocarcinoma)
Observed bands are 33,42, 55, 100, unsure as to the identity of these extra bands
Lane 3-8 CLL isolated B-lymphocytes
Lane 9-10 T47D ( (Human ductal breast epithelial tumor cell line)
Lane 11-12 SW837 (Human Caucasian rectum adenocarcinoma)
Observed bands are 33,42, 55, 100, unsure as to the identity of these extra bands
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Elena Kashuba
Verified customer
投稿 Apr 10 2013