Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Uterus)
Permeabilization
Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification
Uterus
Blocking step
3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA / 3% donkey serum in PBST
Concentration
5 µg/ml
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Image shows frozen section of adult mouse uterus. Kindlin-positive staining was detected in cells in the epithelial lining of the uterus. Image shows DAPI counterstain, red field (autofluorescence), antibody staining and overlay.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 21 2015