Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Lung Epithelial cells)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Lung Epithelial cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBSA
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Goat anti Rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
The image is ki67 (alexa 488) and dapi (blue) many of the nuclei cells positive (green staining) for example the cell in the centre rigjt of the image has bright green staining.
Abcam response
no green color, though secondary is green in color
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 22 2018