Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Spleen
Fixative
Paraformaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 20°C · Diluent: Dilution Buffer from Dako with blocking
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Envision anti-rabbit from Dako
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Rudolf Jung
Verified customer
投稿 Mar 14 2016