Anti-Kallikrein 1 抗体 - Kallikrein loop (ab28289)

製品の概要

  • 製品名
    Anti-Kallikrein 1 antibody - Kallikrein loop
    Kallikrein 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Kallikrein 1 - Kallikrein loop
  • 特異性
    ab28289 recognises the Kallikrein loop of Kallikrein 1. It does not react with the other human Kallikreins.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to the the Kallikrein loop area of human Kallikrein 1. (Peptide available as ab41419.)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • 組織特異性
    Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • 配列類似性
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾
    The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glandular kallikrein 1 antibody
    • hK 1 antibody
    • hK1 antibody
    • Kallikrein serine protease 1 antibody
    • Kallikrein-1 antibody
    • Kidney/pancreas/salivary gland kallikrein antibody
    • Klk 6 antibody
    • KLK1 antibody
    • KLK1_HUMAN antibody
    • Klk6 antibody
    • KLKR antibody
    • Tissue kallikrein antibody
    see all

画像

  • All lanes : Anti-Kallikrein 1 antibody - Kallikrein loop (ab28289) at 1/1000 dilution

    Lane 1 : Kalikrein 1 at 0.05 µg
    Lane 2 : Kalikrein 1 at 0.01 µg
    Lane 3 : Kalikrein 1 at 0.001 µg


    Predicted band size : 28 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

参考文献

This product has been referenced in:
  • Tang SC  et al. Bradykinin and high glucose promote renal tubular inflammation. Nephrol Dial Transplant 25:698-710 (2010). ICC/IF ; Human . Read more (PubMed: 19923143) »
  • Cassim B  et al. Kallikreins, kininogens and kinin receptors on circulating and synovial fluid neutrophils: role in kinin generation in rheumatoid arthritis. Rheumatology (Oxford) 48:490-6 (2009). ICC/IF, IHC ; Human . Read more (PubMed: 19254919) »

See all 2 Publications for this product

レビューと Q&A

Following up my previous message, immunogens for the kallikrein antibodies ab28289 and ab40497 were aligned with the mouse canonical sequence (http://www.uniprot.org/uniprot/P15947.fasta). The ab28289 epitope falls in a region of relatively low seq...

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Thank you for contacting us. I am still waiting for our source for the kallikrein antibodies ab28289 and ab40497 to send their alignments of the immunogens and mouse kallikrein but I have checked the homology of the immunogens for the other four ant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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