Anti-KPNA2 抗体 (ab84440)
Key features and details
- Rabbit polyclonal to KPNA2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KPNA2 antibody
KPNA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KPNA2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Orangutan -
免疫原
Synthetic peptide corresponding to a region within C terminal amino acids 475-529 (ALQNHENESV YKASLSLIEK YFSVEEEEDQ NVVPETTSEG YTFQVQDGAP GTFNF) of human KPNA2 (NP_002257.1).
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特記事項
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84440の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
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特記事項 |
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IHC-P
1/100 - 1/500. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
組織特異性
Expressed ubiquitously. -
配列類似性
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
ドメイン
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3838 Human
- Entrez Gene: 16647 Mouse
- Omim: 600685 Human
- SwissProt: P52292 Human
- SwissProt: P52293 Mouse
- Unigene: 594238 Human
- Unigene: 12508 Mouse
- Unigene: 391577 Mouse
see all -
別名
- IMA1_HUMAN antibody
- Importin alpha 1 antibody
- Importin alpha 2 antibody
see all
画像
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Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human metastatic lymph node tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human bladder carcinoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse squamous cell carcinoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse teratoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of Human ovarian germ cell tumour, staining KPNA2 with ab84440.
Antigen retrieval was performed by submerging the sample in citrate buffer (pH 6) and microwaving. The sections were blocked with 1% BSA before incubating with primary antibody (1/400). Staining was detected using DAB.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab84440 は 26 報の論文で使用されています。
- Miyamoto Y et al. SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication. Commun Biol 5:483 (2022). PubMed: 35590097
- Dong L et al. MiR-101-3p targets KPNA2 to inhibit the progression of lung squamous cell carcinoma cell lines. Histol Histopathol N/A:18573 (2022). PubMed: 36583484
- Zhou KX et al. Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC. J Immunol Res 2021:6694392 (2021). PubMed: 33728352
- Zeng F et al. KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. J Transl Med 19:112 (2021). PubMed: 33731128
- Fan X et al. Silencing of KPNA2 inhibits high glucose-induced podocyte injury via inactivation of mTORC1/p70S6K signaling pathway. Biochem Biophys Res Commun 521:1017-1023 (2020). PubMed: 31727365