Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (lung cancer line LKR10 and LKR13)
Specification
lung cancer line LKR10 and LKR13
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Amersham-HRP sheep anti-rabbit
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: ca. 50 kDa
Exposure
15 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 25 2014