Anti-ASH1L 抗体 [ASH5H03] (ab50981)
Key features and details
- Mouse monoclonal [ASH5H03] to ASH1L
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-ASH1L antibody [ASH5H03]
ASH1L 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ASH5H03] to ASH1L -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Human -
免疫原
Recombinant fragment corresponding to Human ASH1L aa 1611-1728.
Sequence:IGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLNRLHRKESLPSNERAVQTL AGSQPTSDKPSQRPSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASG DDSVDSLLQRMVQNEDQE
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab50981 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum -
ポリ/モノ
モノクローナル -
クローン名
ASH5H03 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50981の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
特記事項 |
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WB: Use at an assay dependent dilution. Predicted molecular weight: 330 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Histone methyltransferase. Probably methylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation. -
組織特異性
Widely expressed, with highest level in brain, heart and kidney. -
配列類似性
Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 3 A.T hook DNA-binding domains.
Contains 1 AWS domain.
Contains 1 BAH domain.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 post-SET domain.
Contains 1 SET domain. -
細胞内局在
Nucleus. Cell junction > tight junction. Chromosome. The relevance of tight junction localization is however unclear. - Information by UniProt
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参照データベース
- Entrez Gene: 55870 Human
- Omim: 607999 Human
- SwissProt: Q9NR48 Human
- Unigene: 491060 Human
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別名
- Absent small and homeotic disks protein 1 homolog antibody
- Absent Small or Homeotic Like antibody
- Absent Small or Homeotic Like antibody
see all
画像
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Anti-ASH1L antibody [ASH5H03] (ab50981) + Recombinant ASH1L (immunogen)
Predicted band size: 330 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab50981 は 3 報の論文で使用されています。
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Atta H et al. Mutant MMP-9 and HGF gene transfer enhance resolution of CCl4-induced liver fibrosis in rats: role of ASH1 and EZH2 methyltransferases repression. PLoS One 9:e112384 (2014). Dot blot . PubMed: 25380300
- Perugorria MJ et al. Histone methyltransferase ASH1 orchestrates fibrogenic gene transcription during myofibroblast transdifferentiation. Hepatology 56:1129-39 (2012). WB . PubMed: 22488473