Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4%PFA
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: donkey Anti-Rabbit IgG (H+L)711-095-152 Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/200
Additional data
Additional Notes
wenqing@ioz.ac.cn
Division of Reproductive Biology,
State Key Laboratory of Reproductive Biology,
Institute of Zoology, Chinese Academy of Sciences
Room D-215, 1 Beichen West Road, Chaoyang District Beijing 100101, China
Abcam response
Mouse species has not been tested with this antibody. We have mixed set of data received from our customers, some lot worked some doesn't so in-order to avoid any confusion we no longer guarantee this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Qing Wen
Verified customer
投稿 May 01 2014