Anti-KLF4 抗体 (ab106629)
Key features and details
- Rabbit polyclonal to KLF4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-KLF4 antibody
KLF4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KLF4 -
由来種
Rabbit -
特異性
ab106629 will detect all three known isoforms. It does not cross-react with other KLF members. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human KLF4 (C terminal). 20 amino acid synthetic peptide located near the c-terminus.
Database link: O43474 -
特記事項
For information on sample preparation for western blot, please refer here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab106629 is purified by affinity chromatography via a peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106629の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa. |
ターゲット情報
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機能
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. -
配列類似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
ドメイン
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9314 Human
- Omim: 602253 Human
- SwissProt: O43474 Human
- Unigene: 376206 Human
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別名
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
see all
画像
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Lane 1 : Anti-KLF4 antibody (ab106629) at 1 µg/ml
Lane 2 : Anti-KLF4 antibody (ab106629) at 2 µg/ml
All lanes : Human liver tissue lysate (7.5 µL (2 mg/mL) per well)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG-HRP conjugated secondary at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 30 secondsRun in running buffer at 130V for 2hours(or until the dye front is close to the bottom);
Transfer the proteins to the membrane at 250mA in transfer buffer for 2hours.
After transfer, wash membrane in 1xTBST.
Incubate the membrane with blocking buffer overnight at 4°C with gentle agitation.
Dilute ab106629 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab106629 overnight at 4oC. Wash 3 times x 10 minutes in washing buffer with gentle agitation.
Dilute 1μL anti-rabbit IgG-HRP conjugated secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 hour at room temperature. Wash 3 times x 10 minutes in washing buffer with gentle agitation.
Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS.
Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS.
Blocking Buffer: 5% non-fat dry milk, TBS.
Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling KLF4 with ab106629 at 5 µg/mL. Antigen Retrieval: Use Citrate buffer antigen retrieval using a microwave. Endogenous peroxide blocking with 3% H2O2. Blocking with 5% BSA at room temperature for 30 minutes. ab106629 incubation at 4°C overnight. Secondary antibody incubation at room temperature for 30 minutes. Washing 3 times for 5 minutes using phosphate buffered saline. Detection using DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab106629 は 39 報の論文で使用されています。
- Xia KS et al. An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration. Bioact Mater 21:69-85 (2023). PubMed: 36017070
- Myint K et al. Oncofetal protein IGF2BP1 regulates IQGAP3 expression to maintain stem cell potential in cancer. iScience 25:105194 (2022). PubMed: 36217548
- Jia X et al. DUB3/KLF4 combats tumor growth and chemoresistance in hepatocellular carcinoma. Cell Death Discov 8:166 (2022). PubMed: 35383144
- Ai Z et al. Krüppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency. Cell Rep 40:111240 (2022). PubMed: 36001968
- Xu D et al. KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury. Acta Pharmacol Sin 42:436-450 (2021). PubMed: 32647339