Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-20% TG)
Sample
Mouse Tissue lysate - whole (retina and brain)
Specification
retina and brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Jackson ImmunoResearch peroxidase-conjugated Affin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
ECL
Bands
Specific: 90 kDa
Exposure
2 minute(s) and 0 second(s)
Negative control
spleen - not included in picture
Additional data
Additional Notes
ABTRIAL-TV7ES
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 07 2015