Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3 cells)
Permeabilization
No
Specification
NIH-3T3 cells
Blocking step
1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey Serum + 1% BSA + 0.1% Triton X
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
NIH-3T3 cells were serum starved for 24 hours prior to staining.
Kif3a staining appears punctate cytoplasmic and possibly enriched in primary cilia.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 02 2015